Assessment of Antiurolithic Potential of Crude Extracts of Berberis lycium by In-vitro Inhibition of Calcium Oxalate Crystallization


Page No: 30-38

Keywords: Urolithiasis, Calcium oxalate (CaOx), Berberis lycium, inhibition assay, nucleation assay, aggregation assay, Tris buffer, crystallization

Abstract: Developing non surgical methods for removal and prevention of reoccurrence of urolithiasis is challenge for researchers of the present time. Traditional systems of medicine utilize teas, decoction or powdered plant parts for removal of kidney stones. Berberis lycium is among the plants which find its traditional use in urolithiais along with other therapeutic effects. Crude aqueous and crude methanol extracts of dried root bark of the plant were studied at 10μg/ml, 50μg/ml, 100μg/ml and 1000μg/ml concentrations for inhibition assay, nucleation assay and aggregation assay. Assays were carried out at 37°C in the presence of buffer containing Tris uffer containing Trisuffer containing Trisuffer containing Tris uffer containing Trisuffer containing Tris uffer containing Trisuffer containing Trisuffer containing Tris –HCl HCl 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.50.05 mol/l and NaCl 0.15 l, pH 6.50.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.50.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 0.05 mol/l and NaCl 0.15 l, pH 6.5 . Both extracts had significant in vitro antiurolithic activity but methanol extract of the plant was found more effective in preventing crystallization in all the three assays. Thus, Berberis lycium can serve as Potential source of active ingredient for antiurolithiais treatment agents.

By: Zahid Munawar & Mateen Abbas

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